
中文名称:CellaQuant™- 大鼠白介素 4(IL-4)酶联免疫吸附测定试剂盒
货号:CQR007(曾用货号:CQR007、GECQR007)
规格:96 T、48 T、96 T×5
反应性:Rat
检测范围:15.63-1000 pg/mL
灵敏度:7.85 pg/mL
样本类型:细胞培养上清液、细胞裂解液、其他生物体液
样本体积:100 μL
精密度:板内、板间变异系数均<10%
回收率:80%-120%
储存条件:2-8℃
有效期:12 个月
Elabscience CellaQuant™-大鼠白介素4(IL-4)elisa试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-4抗体包被于酶标板上,实验时样品(或标准品&质控品)中的大鼠IL-4会与包被抗体结合。后依次加入生物素化的抗大鼠IL-4抗体和辣根过氧化物酶标记的亲和素,抗大鼠IL-4抗体与结合在包被抗体上的大鼠IL-4结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-4浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-4的浓度。
细胞样本适配性好:专为细胞上清与细胞裂解液优化,经充分验证,背景干扰低;
检测性能稳定可靠:灵敏度达 7.85 pg/mL,检测范围覆盖 15.63-1000 pg/mL,精密度良好;
特异性表现良好:可特异性检测大鼠 IL-4,与其他类似物无明显交叉反应;
批间一致性优异:参照临床质控标准开发,核心参数可溯源,结果可复现;
操作便捷高效:整体反应时长 3 小时 30 分钟,适配常规实验室酶标仪,易上手。
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